In case of blood cell counting, the squares placed at the corners are used for white cell counting. The grid has 9 square subdivisions of width 1mm (See Fig.

Neubauer chamber's counting grid is 3 mm x 3 mm in size. The chamber has three parts: (1) the central part, where the counting grid has been set on the glass, and (2) double chambers/two counting areas that can be loaded independently. In a simple counting chamber, the central area is where the cell counts are performed. The Neubauer chamber is a thick crystal slide with the size of a glass slide (30 x 70 mm and 4 mm thickness). Equipment necessary for performing cell count with hemocytometer Hemocytometer or Neubauer chamber Pipette/Micropipette with disposable tipsįigure 1.Materials The materials needed to perform a cell count with Neubauer chamber are as follows: The technical data sheet describes the best practices, applications, and recommendations when performing a cell count. The principles described in this technical data sheet apply to any cell counting chamber, although the dimensions and volumes of each chamber may differ. This technical data sheet has been composed in order to help experienced, as well as non-experienced, researchers perform a proper cell counting using a Neubauer chamber or Hemocytometer. Neubauer Haemocytometry EMS Catalog #68052-14, 68052-15 Introductionĭespite the fact of the recent technical development of scientific laboratories, the Neubauer chamber remains the most common method used for cell counting around the world.

Burker Turk Counting Chamber Manual